Abstract:With 95%ethanol as the extraction solvent，the effective components in 11 samples Phellinus igniarius（P.igniarius）from different sources were extracted，and the contents of davallialactone，hypholomine B and inoscavin A in the extracts were detected by（high performance liquid chromatography）HPLC.The results showed that davallialactone，hypholomine B and inoscavin A had good linearity in the range of 2.5 μg/mL-10 μg/mL（r=0.998 9，0.998 1，0.999 9），and the precision relative standard deviation（RSD）was 0.49%，0.57%and 0.75%，respectively.The stability RSD was 0.67%，0.66%，and 0.73%，and the reproducible RSD was 0.62%，0.58%，and 0.41%（n=5）.The method is excellent in precision，stability and repeatability.By comparing the content of three compounds in the extracts of 11 different types of Phellinus igniarius，it was found that the content of three compounds in the extracts of different types of Phellinus igniarius was significantly different，which may be related to the growth environment，growth year and cultivation mode of Phellinus igniarius.This study provided a certain experimental basis for the quality control of medicinal Phellinus igniarius and the development of related drugs.