Abstract:To explore the optimum conditions for extracting pancreatic lipase from frozen pig pancreas.The enzyme activity was used as the evaluation index.The optimum extraction conditions of pancreatic lipase were obtained by single factor test and orthogonal experiment，and the pancreatic lipase was concentrated with dialysate bag.Finally，the stability of pancreatic lipase solution was studied from two aspects：compound stabilizer and preservation temperature.The optimum extraction conditions were as follows：extraction with sodium chloride solution of 0.15 mol/L，temperature 25℃，pH 7.5；The stability of the liquid enzyme was poor.By exploring the influence of the compound stabilizer on the stability of pancreatic lipase，the optimum formula of the compound stabilizer was 1.0%glycerol，1.0%Tween 80，1.2%sodium chloride and 2.2%calcium gluconate.With 1.2%maltose and 1.2%soluble starch，the optimum storage temperature was 25℃.The enzyme activity reached（194 715.67±3 774.03）U/g after 24 h reverse osmosis at 4 ℃.