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食品研究与开发:2020,41(16):133-138
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复合酶制备柠檬膳食纤维工艺条件优化
(1.内江师范学院化学化工学院,四川内江641199;2.果类废弃物资源化省高校重点实验室,四川内江641199)
Optimization of Dietary Fiber Extraction from Lemon by Compound Enzyme
(1.College of Chemistry and Chemical Engineering,Neijiang Normal University,Neijiang 641199,Sichuan,China;2.Key Laboratory of Fruit Waste Treatment and Resource Recycling of the Sichuan Provincial College,Neijiang 641199,Sichuan,China)
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投稿时间:2019-08-10    
中文摘要: 为提高膳食纤维质量,研究木瓜蛋白酶及α-淀粉酶制备柠檬皮渣中膳食纤维的工艺。考察木瓜蛋白酶及α-淀粉酶酶浓度、料液比、制备时间、缓冲液pH值、制备温度对膳食纤维得率和质量的影响,结合正交试验优化复合酶制备柠檬膳食纤维的最佳工艺条件:制备时间30 min,温度60℃,pH 6.0,料液比1∶20(g/mL),木瓜蛋白酶用量0.8mg/g,α-淀粉酶用量2.4mg/g。最佳条件下可溶性膳食纤维得率为(28.2±0.6)%,不溶性膳食纤维得率为(42.2±1.2)%,可溶性膳食纤维占总膳食纤维的(39.8±1.6)%。
Abstract:Lemon dietary fiber was extracted by papain and alpha amylase.The effects of enzyme concentration of papain and alpha amylase,solid-liquid ratio,extraction time,pH,temperature on dietary fiber yield were investigated and the optimum process conditions of compound enzyme extraction was optimized by orthogonal experiment:extraction time of 30 min,temperature of 60 ℃,pH of 6.0,solid-liquid ratio of 1 ∶20(g/mL),papain of 0.8 mg/g,alpha amylase of 2.4 mg/g.The rate of water-soluble dietary fiber was (28.2±0.6)%,the rate of insoluble dietary fiber was(42.2±1.2)%,water-soluble dietary fiber was achieved(39.8±1.6)%in total dietary fiber under best conditions.
文章编号:202016021     中图分类号:    文献标志码:
基金项目:四川省教育厅成果转化培育项目(14CZ0025)
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