Abstract:In order to extract crude polysaccharides in the leaves of Moringa oleifera and study its in-vitro antioxidation activity，on the basis of the single factor extraction experiment，this research used ethanol subsiding method to get crude polysaccharides in the leaves of Moringa oleifera，optimized techniques of polysaccharide extraction with orthogonal experiment and analyzed in-vitro antioxidation activity of crude polysaccharide of the leaves of Moringa oleifera in 6 areas.The research showed that the best extracting processes were as follows，liquid to solid ratio was 20∶1（mL/g），extracting temperature was 70℃ and extracting time was 1.5 h.Under such conditions，the final productivity of polysaccharides was up to 11.48%.The in-vitro antioxidation activity of crude polysaccharide of the the leaves of Moringa oleifera in Puer was the best among 6 areas.