Abstract:There is growing evidence that probiotics regulate brain function and behavior through the microbiota-gut-brain axis.In this study，three strains of lactic acid bacteria with antidepressant/anxiety-like behavioral potential were selected from the probiotics library of our laboratory by using the behavior model.The effects of Lactobacillus on anxiety-like behavior in healthy mice were studied by light and dark box and elevated plus-maze test.The effects of lactic acid bacteria on depression-like behavior in healthy mice were studied by tail suspension test and forced swimming test.The study showed that four weeks after supplementation of Lactobacillus kefiranofaciens ZW3，Lactobacillus plantarum MA2 and Lactobacillus plantarum 299（108 CFU），compared to blank control mice，the residence time in the bright box in the light and dark box and the residence time in the open arm in the elevated plus-maze test could be increased，and the anxious behavior of mice could be improved.In addition，these three strains could also reduce the immobile time of mice in tail suspension test and forced swimming test，and improve the depression-like behavior of mice.They also reduced serotonin levels and serum corticosterone levels in brain biomarkers.These results suggested that Lactobacillus kefiranofaciens ZW3，Lactobacillus plantarum MA2 and Lactobacillus plantarum 299，as probiotics，had the potential to improve anxiety-like and depression-like behaviors.