Abstract:The total phenolic acid contents in Lycium barbarum L. were determined by the Folin-Ciocalteu method. The ultrasonic-assisted extraction conditions were optimized.The material liquid ratio，extration solution concentration and extration time were optimized by single factor method.The results showed that extration solution was 70%，the ratio of solid to liquid 1∶30（g/mL），extration time 90 min.The polyphenols content which thirty Lycium barbarum L. samples in four different varieties of four producing area in Ningxia were analyzed. The results showed that the total phenolic acid contents between 4.53 mg/g and 16.07 mg/g，which the content of summer fruit were 4.53 mg/g-14.57 mg/g and the content of autumn fruit were 11.60 mg/g-16.07 mg/g. The content of autumn fruit was higher and 1.1-2.6 times of summer fruit.There were significant differences in total phenol content of the same variety in different producing areas.