Abstract:In this research，the spray drying process was optimized by response surface method，and the aroma was qualitatively analyzed by gas chromatography-mass spectrometer（GC-MS）.The mice were divided into three groups：low，medium and high dose groups of Epimedium brevicornu and Coreopsis tinctoria instant tea and control group，the weight-bearing swimming test，serum urea test，hepatic glycogen and muscle glycogen test were performed after 30 days of continuously giving test substances for them.The best spray drying process was：inlet temperature 153.00℃，β-cyclodextrin addition 8.00%，charging rate 10.00 mL/min，powdering rate（71.37±0.832）%.A total of 44 aroma components were detected in 10 kinds of Epimedium brevicornu and Coreopsis tinctoria instant tea，accounting for 32.19%of the total sealing area.In the evaluation of alleviating physical fatigu，the exhaustion time in the middle and high dose group of mice was significantly higher than that in the blank group （P＜0.01），the blood urea nitrogen content was significantly lower than that in blank group（P＜0.01），the hepatic glycogen and muscle glycogen content in mice were significantly higher than those in the blank group（P＜0.01）.It indicateed that Epimedium brevicornu and Coreopsis tinctoria instant tea had the function of alleviating fatigue in mice.