Abstract:The enrichment process of total anthocyanins crude extract from Lycium ruthenicum Murr.with sephadex LH-20 was investigated.The influence of target object adsorption of different sample concentration， adsorption flow rate，and sample pH value were reseached.At the same time，the influence of anthocyanins recovery of different ethyl alcohol concentration，elution flow rate，and eluent pH value were investigated.The best purification conditions by single factor and orthogonal test showed that pH 3.5 sample solution with anthocyanins concentration of 25 mg/L，adsorption velocity 1.0 mL/min，eluent ethanol concentration of 85%，eluent pH of 3.5，elution velocity of 1.0 mL/min，the adsorption rate and desorption rate of anthocyanins was 90.9%，85.3%respectively.The total anthocyanins content of sample after purification was obviously higher than the resin purification product，Moreover，the results from two antioxidant assays consistently indicated that the antioxidant capacity of product by gel purification，resin purification and microwave-assisted revealed a decreasing trend in turn.