Abstract:Flumequine（FLU），an animal-specific antibiotic，was abused or over standard used and resulted in the residual problem in animal foods，which caused great harm to human health.Complete antigen of FLU was prepared by active ester method and was used to immunize New Zealand white rabbits to obtain antiserum.The titer and affinity of the antiserum were determined by indirect competitive enzyme linked immunosorbent assay（ELISA）.The titer was 1 ∶12800 and IC50 was 0.055 ng/mL after purification.Colloidal gold was prepared by trisodium citrate reduction method and immune gold was prepared by FLU antibody being labeled with colloidal gold.Nitro-cellulose membrane strip which used as solid carrier was separately coated with coating antigen（as test line）and enzyme-labeled secondary antibody （as control line）.The optimum conditions were as follows：blocking solution was 5 % skimmed milk，enzyme-labeled antibody dilution times was 40 times，coating amount was 1.0 μg，immunogold dilution was 1 ∶5 （volume ratio），the mixing ratio of gold-labeled antibody and the solution to be tested was 1 ∶5 （volume ratio）.Under the optimum conditions，the FLU colloidal gold labeled immunochromatographic strip was prepared with a minimum detection limit of 40 μg/L.The established solid phase membrane-based immunoassay was suitable for rapid qualitative screening.