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投稿时间：2019-02-21
投稿时间：2019-02-21
中文摘要: 研究酱香型白酒中非乙醇物质（maotai-flavor liquor extract，MTE）的体外抗炎活性及其作用机制。采用氯仿萃取及真空冻干获得MTE，液相色谱-质谱联用技术（high performance liquid chromatography-mass spectrometry，HPLCMS）分析其成分，脂多糖（lipopolysaccharide，LPS）诱导IEC-6 细胞建立炎症模型；检测MTE 及MTE 中26 种化合物对LPS 诱导的IEC-6 细胞凋亡的影响；酶联免疫吸附测定（enzyme linked immunosorbent assay，ELISA）测定MTE 及α-雪松醇处理细胞对NO 及相关炎症因子IL-6、IL-8、TNF-α、IL-10 及细胞迁移能力的影响。结果表明，MTE、α-雪松醇（0.22 mg/mL）可显著提高IL-10 分泌、降低LPS 诱导的细胞凋亡、提高细胞迁移能力，降低IL-6、IL-8、TNF-α 等的分泌，上调细胞紧密连接蛋白Occludin 和ZO-1 mRNA 表达。酱香型白酒中含有的非乙醇物质具有一定的抗炎活性，其中α-雪松醇可显著降低LPS 诱导引起的IEC-6 细胞凋亡，可通过抑制炎症因子IL-6、IL-8 和TNF-α 的分泌及提高IL-10 分泌发挥抗炎能力。
Abstract:To study the anti-inflammatory activity and mechanism of Maotai-flavor liquor extract（MTE）in vitro.Isolated the MTE by extraction and lyophilization and analyed it by HPLC-MS.An inflammation model was established in IEC-6 cells with lipopolysaccharide（LPS）simulation then the anti-inflammatory effects of MTE were detected.Results showed that MTE and α-cedrol could significantly increase the secretion of IL-10，decrease apoptosis，enhance cell migration ability，decrease the secretion of IL-6，IL-8，TNF-α，and upregulate the gene expression of Occludin and ZO-1.Results showed that the bioactive compounds in Maotaiflavor liquor can significantly reduce the apoptosis of IEC-6 cells induced by LPS and exert anti-inflammatory ability by inhibiting the secretion of inflammatory factors and increasing the secretion of IL-10.
文章编号：202003003 中图分类号： 文献标志码：
基金项目:深圳市重点科技项目（JCYJ20140418095735538）
| Author Name | Affiliation |
| CAO Hui，LIU Jie，LIU Zhi-gang | Institute of Allergy and Immunology，Shenzhen University，Shenzhen 518055，Guangdong，China |
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