Abstract:Using Urtica fissa Pritz.as raw material，it was extracted with 70%ethanol，taking the extract as the research object，the content of flavonoids was determined.To evaluate the antioxidant capacity，taking VC as a positive control，the 1，1-diphenyl-2-trinitrophenylhydrazine radical （DPPH·）scavenging rate，hydroxyl radical （·OH）scavenging rate and total reducing power were determined.The inhibitory effect of the Urtica fissa Pritz.extract on α-glucosidase was determined by using p-nitrophenyl-α-D-glucopyranoside（PNPG）as a substrate.The results showed that the flavonoid content in the ethanol extract of Urtica fissa Pritz.was 12.09 mg/g；the maximum clearance rate to DPPH free radical was 92.76 %，and the semi-inhibitory concentration （IC50）was 9.960 μg/mL，the maximum clearance rate to hydroxyl radicals was 98.05%，the IC50 value was 1.123 mg/mL，and the total reducing power increased with the increase of alcohol extract concentration；the maximum inhibition rate of α-glucosidase was 59.06 %，and the IC50 value was 31.598 mg/mL.The results showed that the ethanol extract of U.fissa Prtz.had clear antioxidant activity in vitro and α-glucosidase inhibitory effect，and it is expected to be developed into an antioxidant hypoglycemic functional food.