Abstract:The quick determination method of benzo（a）pyrene（BaP）in smoked seafood by on-line solid phase extraction -high performance liquid chromatography was investigated.Smoked seafood samples could be analyzed directly after extraction with ethyl acetate.After purification by on-line purification column，and separated by C18 column，then detected by fluorescence detector.The detection limit of BaP was 0.035 ng/mL.The correlation coefficient was 0.999 9，the recoveries of the anions were in the range of 89.6 %-99.0 %，the relative standard deviations of fluoride was 1.3%-3.1%。