Abstract:In order to provide reference for solid fermentation of Cordyceps，2 strains of Cordyceps were inoculated into 5 kinds of solid medium with wheat as the main component and adding different proportions of accessories，active ingredients and main nutritional components of different fungal substance were determined.The results showed that the polysaccharide content of the same fungal substance after starch removal was lower than that before starch removal，starch removal could reduce the interference of starch in fungal substance on the determination of Cordyceps polysaccharides.ZNB2.1 and ZNB2.2 produced the most Cordyceps polysaccharides on B and E medium，respectively，the ability of ZNB2.1 to produce polysaccharides was greater than that of ZNB2.2.After fermentation with two strains，the reduced sugar content of substrates increased and the starch and protein content decreased.ZNB2.2 could produce cordycepin and cordycepic acid，the contents of cordycepin and cordycepic acid in fungal substance reached 207.4 mg/kg and 22.1 g/kg，respectively.Cordycepin and cordycepic acid were not detected in ZNB2.1 fungal substance.Considered the yield of Cordyceps polysaccharide，cordycepin and cordycepic acid，ZNB2.2 strain and E medium were suitable for solid-state fermentation of Cordyceps.