Abstract:For investigation the effect of extraction technology on the extraction rate of deer oil，the supercritical CO2 extraction method was used to extract deer oil from deer（Cervus Nippon）fat.Deer fat used as material for extracted the deer oil，the extraction temperature，extraction pressure，extraction time and flow rate of CO2 were investigated by single factor experiment and orthogonal experiment in deer oil extraction.The results showed that the best extraction process determined as extraction temperature as 45 ℃，extraction pressure as 40 MPa，extraction time as 150 min，CO2 flow rate as 14 L/h and the extraction percentage of deer oil was 44.81 %.The components of deer oil were detected by gas chromatography mass spectrometry（GC-MS），the results suggested that 16 kinds of fatty acids was in oil，the relative contents of saturated fatty acids in deer oil was 69.840 9%and unsaturated fatty acids was 30.159 1%.The main composition of fatty acids in deer oil were palmitic acid，octadecanoic acid and heptacosic acid，according to the nature of various fatty acids.