Abstract:This study aimed to isolate and screen lactic acid bacteria with great fermentation performance from traditional fermented blueberry beverages，which provided a theoretical basis for deep processing of blueberry.High-throughput sequencing of the bacterial 16S rDNA V4 region in the sample was performed using Ion S5 XL sequencing platform.Lactobacillus was the main fermentation genus，with a relative abundance of 69.1 %.50 lactic acid bacteria were obtained by separation and identification.Compared with 16S rDNA data，the se－quence similarity between 8 strains and the highest ranked lactic acid bacteria clustering unit was 100%.Two strains of Lactobacillus plantarum were obtained through further producing acid and tolerance test，and growth state in fermented blueberry juice.The strains were TUST-L4 and TUST-L27，which were suitable for the development of lactic acid bacteria fermented blueberry beverage.