Abstract:Salicornia Europaea L.seeds were used as experimental materials，germination rate was used as an index to determine the level of factors by single-factor test.Then using the germination rate，γ-aminobutyric acid （GABA）and flavonoids content as indicators，the enrichment conditions were optimized by orthogonal experiments.Under the optimal conditions，the effects of the changes of the activities of key enzymes on the enrichment of active substances were investigated.The results showed that the optimal germination and enrichment conditions were as follows：NaCl concentration was 7.5 g/L，pH was 8，GA3 concentration was 5 mg/L and cultivation temperature was 24 ℃ .Under these optimal conditions，the activities of glutamate decarboxylase（GAD）and Diamine oxidase（DAO）increased at first and then decreased，which promoted the accumulation of GABA.On the fifth day，the GABA content reached the maximum value of 310.26 mg/100 g，which was 3.5 times higher than that of ungerminated seeds.The activities of phenylalanine aminolyase（PAL），cinnamicacid-4-hydroxylase （C4H）and 4-coumarate-CoA ligase （4CL）increased at first and then decreased，which was consistent with the change trend of flavonoids.On the fifth day，the content of flavonoids reached the maximum of 31.76 μg/mL，which was 2.0 times higher than that of ungerminated seeds.