Abstract:Using foodborne pathogens bacterial Staphylococcus aureus-Escherichia coli-Salmonella and their mixed bacteria as the object，the optimal conditions for the formation of mixed biofilm were studied by the single factor analysis and Box-Behnken test.The effect of tryptone soy broth（TSB），glucose and NaCl on the biofilm formation ability of single bacteria and mixed bacteria was determined by 96 microplate method.The results showed that the optimum culture condition for mixed bacterial biofilm was pH 7，at 36 ℃ for 22 h.When the TSB medium with 1.6 % mass fraction was used，the biofilm formed by the pathogen under the optimal conditions had the highest adhesion rate；the carbon source promoted the formation of biofilm；when the mass fraction of NaCl was more than 0.4%，the ability to form biofilms of foodborne pathogens was inhibited.In summary，the formation of the pathogen biofilm was affected by the culture conditions.