Abstract:In order to improve the added value of octopus visceral，this study selected the suitable organic solvent as extraction solvent，and the ratio of material to liquid of octopus viscera，temperature and extraction time were optimized for ultrasonic extraction of visceral fat in octopus by single factor test，Box-Behnken test principle and response surface methodology. Results showed that the optimal extraction conditions for the extraction of visceral fat were：ultrasonic power 180 W，auxiliary extraction time 30 min，ratio of material to liquid 1 ∶8 （g/mL），extraction temperature 56 ℃，extraction time 2.6 h，the best extraction rate 81.12%.This study was of great significance for the deep processing and reuse of octopus viscera.