Abstract:The regulating effect of berberine on adipose tissue fibrosis and intestinal microflora in obese mice was studied.Forty KM mice were randomly divided into normal control，fat model，low concentration and high concentration groups respectively.Mice were given high fat emulsion for 12 weeks to establish model.Then corresponding drugs were given for 9 weeks.The expressions of TNF-α，TGF-β1 and AMPK-α1 were detected by western blotting.The intestinal microflora structure was analyzed by enterobacterial repetitive intergenic consensus-polymerase chain reaction （ERIC-PCR）and polymerase chain reaction-denaturing gradient gel electrophoresis （PCR-DGGE）.The expressions of TNF-α and TGF-β1 were inhibited，but AMPK-α1 was improved with berberine administration.In fat model group，intestina microflora was altered，Facklamia iqnava increased，Lachnospiraceae bacterium and Lactobacillus acidophilus decreased.But Lachnospiraceae bacterium and Lactobacillus acidophilus became dominant microflora with berberine administration.The effect of berberine on adipose tissue fibrosis and intestinal microflora in obese mice was not only related to activate AMPK，inhibit TNF-α and TGF-β1，but also regulate intestinal microflora balance and promote probiotics.