Abstract:In order to investigate the enterotoxin production and drug resistance of food-borne Staphylococcus aureus，explore the types and distribution of enterotoxin，and study the drug resistance characteristics，so as to provide a reference for relevant monitoring and early warning.The strains isolated from the daily food inspection in the year of 2016-2017 in the laboratory，the total amount of enterotoxin was detected by full-automatic fluorescence enzyme-linked immunosorbent assay （miniVIDAS）and typing of enterotoxin SEA ～ SEE by ELISA method.The drug sensitivity test was carried out by automatic microbiological identification system（Phoenix M50）.The results showed that 16 strains of Staphylococcus aureus isolated from food produced enterotoxin，and the positive rate was 55.2 %.Moreover，5 strains of food poisoning were all enterotoxin positive.Strains producing 2 or more enterotoxin were 12 strains，accounting for 41.4 %.Common enterotoxin A～E was detected.The most strains producing SEE were 12 strains，accounting for 41.4 %，followed by 11 strains producing SEA.29 strains of foodborne Staphylococcus aureus were treated with gentamicin，tobramycin，penicillin，ampicillin，oxacillin，amoxicillin clavulanic acid，compound neannoxine，clindamycin，erythromycin，Li Fuping，tetracycline.Resistance to various degrees and multiple resistance were found，among which the resistance rate to penicillin and ampicillin were the highest，followed by 82.8 %，followed by erythromycin 44.8 %.In this study，Staphylococcus aureus isolated from food monitor had a high enterotoxin production rate，the main types are SEE and SEA.Moreover，the isolated foodborne Staphylococcus aureus had different degrees of resistance and multidrug resistance.It was suggested to strengthen monitoring from various links and reduced the risk of food safety caused by drug resistant bacteria.