Abstract:To observe the protective effect of peach gum polysaccharide （PGP）on acute ethanol-induced gastric mucosal injury，and to investigate the anti-inebriation effect and the biological mechanism of peach gum polysaccharide combined with epigallocatechin gallate （EGCG）on mice.The gastric mucosa was simulated by dialysis bag in vitro to study the effect of different concentrations of PGP on the inhibition of ethanol diffusion.In the animal experiment，mice were given 60 % edible ethanol for intragastric administration.After different concentrations of EGCG and PGP were given，the mice were observed for the change of drunk tolerance time and hangover time，assessment the gastric mucosal injury，determination of serum ethanol concentration in mice.The contents of alcohol dehydrogenase（ADH）and acetaldehyde dehydrogenase（ALDH）in mouse liver tissues were determined.In vitro and in vivo experiments showed that PGP could significantly inhibit ethanol diffusion，effectively prolong the intoxication time of mice，and shorten the sobering time of drunk mice.The synergistic intervention of PGP and EGCG could significantly improve the gastric mucosal injury induced by high concentration of ethanol in mice，significantly reduce oxidative stress injury of gastric tissue in mice，increase ADH and ALDH activity in liver tissue of mice，and promote ethanol metabolism，thus exerting its anti-drunkenness and anti-alcoholism effect.