Abstract:A method for the simultaneous determination of ten artificial dyes and sweeteners in baked food by ultra-high performance liquid coupled with quadrupole/exactive orbitrap mass spectrometry（UPLC-Q Exactive Orbitrap MS）was established.The samples were extracted with acetonitrile aqueous solution and purified with n-hexane.In the process of quantification and qualification，a full MS/dd-MS2 experiment was adopted in mass spectrometry acquisition，accompanied with the full scan MS date for quantification，as well as the date dependent MS2 product ion spectra for qualification.The quantitative limit of the method was 10 μg/kg-200 μg/kg.The mass accuracy error was less than 3×10-6.The standard solution had good linearity in the range of 1 ng/mL-20 ng/mL or 10 ng/mL-200 ng/mL.The correlation coefficients were all greater than 0.995.The average recovery was 64.5 %-119.5 %.The method was suitable for screening and confirmation of various additives in baked goods.