Abstract:In order to develop a rapid quantitative test strip for the detection of T-2 toxin in corn，the T-2 toxin monoclonal antibody was labeled and the performance of colloidal gold test strip was studied.The T-2 toxin monoclonal antibody was labeled with colloidal gold，and the optimum pH value of the label was determined by measuring the change of the absorbance value after adding different amounts of potassium carbonate.The optimal combination condition was determined by orthogonal experiment with the antigen.The performance of the strip was tested by comparing the detection line and the control line with the colloidal gold quantitative reader.The results showed that the optimal pH value of monoclonal antibody labeling was 8.5，the optimal labeling concentration was 20 μg/mL，the coating concentration of antigen was 0.5 mg/mL，and the coating concentration of goat anti-mouse IgG was 1.0 mg/mL.The detection limit of the strip test T-2 toxin standard was 5 μg/kg.T-2 toxin colloidal gold rapid quantitative test strip test method could obtain concrete data，and there was no cross-reaction with common mycotoxin，the recovery rate was between 77 % and 117.6 %，repetitive detection between batch and batch of variation coefficient was less than 10%，and the detection result of T-2 toxin in corn was consistent with the results of high-performance liquid method.The T-2 toxin colloidal gold rapid quantitative test strip was successfully developed，which can be used for rapid quantitative detection of T-2 toxin content in corn.