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投稿时间:2019-06-20
投稿时间:2019-06-20
中文摘要: 该研究以食源性混合病原菌生物被膜(金黄色葡萄球菌-大肠杆菌-沙门氏菌)为研究对象,研究不同质量浓度的乙二胺四乙酸(ethylenediaminetetraacetic acid,EDTA)对混合菌生物被膜的抑制作用。结果表明,当添加0.25 mmol/L 的EDTA 时即可使混合菌生物被膜黏附率下降16.5%,检测AI-2 分子活性和扫描电镜观察形态进一步证实了随着EDTA 浓度的增加,黏附度逐渐减少;EDTA 仅对处于黏附期(0~28 h)的混合菌生物被膜有抑制作用;添加二价金属离子(Mg2+、Ca2+、Fe2+)可增强混合菌生物被膜的稳定性。
Abstract:In the research,using multi-species biofilm by foodborne bacteria (Staphylococcus aureus-Escherichia coli-Salmonella)as the object,and the inhibitory effects of different concentrations of ethylenediaminetetraacetic acid(EDTA)on the biofilm of mixed bacteria were studied.The results showed that the crystal violet staining method determined that when 0.25 mmol/L EDTA was added,the biofilm adhesion rate of the mixed bacteria decreased by 16.5 %.The activity of AI-2 and the morphology of the scanning electron microscope were further confirmed with the concentration of EDTA.The adhesion was gradually reduced;EDTA only inhibits the mixed biofilm of the mixed phase (0-28 h);the addition of divalent metal ions (Mg2+,Ca2+,Fe2+)could enhance the biofilm of the mixed bacteria stability.
keywords: ethylenediaminetetraacetic acid multi-species biofilm scanning electron microscope quorum sensing metal ion
文章编号:201919010 中图分类号: 文献标志码:
基金项目:内蒙古自治区高等学校科学研究项目(NJZZ17053)
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