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投稿时间：2018-11-16
投稿时间：2018-11-16
中文摘要: 探讨辣木叶提取物对胰脂肪酶的抑制作用，为开发辣木叶天然胰脂肪酶抑制剂提供理论依据。在单因素试验的基础上，用正交试验优化提取条件，研究辣木叶提取物对胰脂肪酶活性的抑制作用，并结合Dixon 图和Lineweaver-Burk 图探讨其抑制类型。结果表明最佳提取条件为：料液比1 ∶50（g/mL）、提取液乙醇浓度70%、超声功率400 W、超声处理时间600 s、提取温度50 ℃；该条件下辣木叶粗提物对胰脂肪酶活力的抑制率为79.15%；辣木叶提取物对胰脂肪酶的抑制作用为非竞争性抑制。辣木叶提取物对胰脂肪酶的活性有明显抑制作用。
Abstract:In order to provide basis for employing Moringa oleifera as lipase inhibitor，the inhibitory effect of extracts of Moringa oleifera leaves on lipase activity was investigated.Based on single-factor experiments，orthogonal experiments were explored to obtain optimum conditions.The effect of the extract of Moringa oleifera on the inhibition of pancreatic lipase activity and the inhibition type Dixon and Lineweaver-Burk graphs were explored.The optimum extracting conditions were established as following：solid to liquid ratio was 1 ∶50（g/mL），ultrasonic power was 400 W，for 600 s with 70%ethanol，at 50 ℃.Under these conditions，the inhibitory rate of extracts of moringa leaves on pancreatic lipase was 79.15 %.The inhibition type was noncompetitive.The extract of Moringa oleifera leaves had inhibitory effect on pancreatic lipase activity.
keywords: Moringa oleifera leaves ultrasonic assisted pancreatic lipase inhibition rate suppression type
文章编号：201919006 中图分类号： 文献标志码：
基金项目:2018 广西自治区级大学生创新创业计划项目（201810594203）
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