Abstract:Methods for determination the content of uronic acid in polysaccharides were reviewed，In order to provide some reference for the accurate determination of the content of uronic acid.By reviewing relevant literatures at home and abroad in recent years，the characteristics of different methods were compared，and analyzed their respective advantages and disadvantages.The common methods were spectroscopy and chromatography.The spectroscopy included pyrazole-sulfuric acid method，hydroxybiphenyl method and 3，5-dimethylphenol method，and the chromatography had gas chromatography，molecular size exclusion liquid chromatography and anionic liquid chromatography.The test conditions and accuracy of different methods were different.The spectroscopy was simple to operate，but there were many interference factors and large deviation and the measured result was the total uronic acid content.The chromatography could accurately determine different kinds of uronic acid content，among which the high-efficiency anion liquid phase chromatography did not require derivatization.It was the primary method for determination the uronic acid content in the future.