Abstract:As a bioactive substance，vitamin B12plays an important role in anemia，nervous system diseases，birth defects，evaluation indicators，nutrition enhancement and so on.Because of the complex structure and lowcontent of vitamin B12，it is of great practical significance to improve its detection methods.This paper reviewed the detection methods of vitamin B12at home and abroad，mainly including liquid chromatography，microbial methods，enzyme-linked immunosorbent assay，atomic absorption spectrometry，inductively coupled plasma assay，electrochemical sensor method，surface plasmon resonance detection，fluorescent molecular probe method，etc.，involving spectroscopy，electrochemistry，immunology，chromatography and microbiology.The technical principle，operation characteristics，analysis effect and application scope of vitamin B12detection method were briefly reviewed.Finally，the development trend of vitamin B12detection technology was discussed and prospected.