Abstract:To study the optimal conditions of banana oligosaccharide on the proliferation of bifidobacterium adolescentis.Using banana oligosaccharide as carbon source，the quantity of bifidobacterium adolescentis as the measure，based on the single-factor experiment，the effect of culture time，additive amount of banana oligosaccharides，initial bacterial concentration and pH of basic medium was studied by the response surface methodology（RSM）.Regression model between the quantity of bifidobacterium adolescentis and the four factors was built.Results showed that banana oligosaccharides as carbon source，the optimal conditions of bifidobacterium adolescentis proliferation was achieved as follows：culture time of 41 h，additive amount of banana oligosaccharides of 4.5 g/L，bacterial concentration of 1.8×106 CFU/mL，pH of basic medium of 7.30.under this condition，three times repeated experiments were carried out，the actual measured number of bifidobacterium adolescentis was 8.6×108 CFU/mL，and the logarithm was 8.965，the predicted relative standard deviation（RSD）value was 0.166%.This experimental model was suitable，the regression equation had good fitting fitness，and the optimal optimization condition was suitable for the proliferation of bifidobacterium adolescentis.