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投稿时间：2018-10-13
投稿时间：2018-10-13
中文摘要: 为筛选产木质素降解酶的菌株，采用苯胺蓝-PDA 平板法和愈创木酚-PDA 平板法从铁皮石斛生长的树皮中筛选到菌株SHIHU-X2，经ITS 测序分析将SHIHU-X2 鉴定为白腐真菌白黄小脆柄菇（Psathyrella candolleana）。应用响应面法优化产漆酶培养基，结果表明SHIHU-X2 菌株产漆酶的最优培养基是：去皮马铃薯200 g/L，葡萄糖20 g/L，蛋白胨 1.24 g/L，KH2PO4 1 g/L，酒石酸铵 0.02 g/L，CuSO4 0.01 g/L，MgSO4 0.56 g/L，MnSO4 0.057 g/L。漆酶活力从初始产酶培养基1.11 U/mL 提高到响应面优化后的2.32 U/mL，漆酶活力提高了109%。
Abstract:In order to screen strains that could degrade lignin，the strain SHIHU-X2 was screened from bark growing from Dendrobium officinale using the aniline blue-PDA plate and guaiacol-PDA plate.By ITS sequencing analysis，SHIHU-X2 was identified as the white rot fungus-Psathyrella candolleana.In order to enhance the concentration of laccase with strain SHIHU-X2，response surface analysis methodology were used to optimize the fermentation medium.Results showed that the optimal culture medium for laccase production by strain SHIHU-X2 were peeled potato 200 g/L，glucose 20 g/L，peptone 1.24 g/L，KH2PO4 1 g/L，ammonium tartrate 0.02 g/L，CuSO4 0.01 g/L，MgSO4 0.56 g/L，MnSO4 0.057 g/L.The ability of laccase production increased from 1.11 U/mL in the initial medium to 2.32 U/mL after the response surface was optimized，and the ability of laccase production increased by 109%.
文章编号：201916031 中图分类号： 文献标志码：
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