Abstract:A novel method for the determination of the pesticide residue in Dendrobium candidum was developed by vortex-assisted dispersive liquid-liquid microextraction （VADLLME）and QuEChERS and Internal standard coupled with gas chromatography-tandem mass spectrometry （GC-MS/MS）.The samples were extracted by acetonitrile solution and purified by N-propyl ethylenediamine （PSA）adsorbent and C18 adsorbent after vortex ultrasound.After purification，1mL acetonitrile was used as dispersant after filtrate filtration membrane，water was added to 4 mL，0.1 mL carbon tetrachloride was extracted and the vortex was mixed，then the emulsion was centrifuged and then centrifuged to absorb the lower extraction liquid.After blow dry，added seven epoxy with internal standard，and constant volume of hexane was added.Determined of GCMS/MS.The factors affecting extraction，enrichment efficiency and purification were optimized.Under the optimized experimental conditions，the concentration of each pesticide could reach 47 times-106 times，the detection limit was 0.353 μg/kg-1.14 μg/kg，the linear range was 0.002 mg/kg-0.5 mg/kg，the correlation coefficient was 0.990 5-0.999 9，the average recovery rate was 77.5 %-97.7 %，and the relative standard deviation was 0.6%-8.4%.