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投稿时间：2018-10-19
投稿时间：2018-10-19
中文摘要: 建立高效液相色谱分析浓缩果汁中游离糖的成分及含量的方法。该方法采用Agilent ZORBAX Carbohydrate色谱柱（4.6 mm×150 mm，5 μm）进行分离，以乙腈-水（75 ∶25）为流动相进行等度洗脱，示差折光检测器检测浓缩果汁中游离糖的成分及含量。结果表明，在0.5 mg/mL～20 mg/mL 范围内，木糖、果糖、葡萄糖、蔗糖、麦芽糖和乳糖的色谱峰面积与其质量浓度呈良好的线性关系（R2＞0.999 0），相对标准偏差为0.77%～4.54%，加标回收率为87.20%～105.22%。
中文关键词: 浓缩果汁 游离糖 高效液相色谱（HPLC） 示差折光检测器（RID） 固相萃取
Abstract:A efficient method for analyzing composition and content of free sugars in concentrated juices based on high performance liquid chromatography（HPLC）was established.The samples were separated on an Agilent ZORBAX Carbohydrate analysis column （4.6 mm×150 mm，5 μm）with the isocratic elution of acetonitrilewater （75 ∶25）as the mobile phases.A differential refraction detector （RID）was used to detect target compounds.Nice linear relationships between the peak areas and mass concentrations of the six sugars were obtained in the range of 0.5 mg/mL to 20 mg/mL with the R2 greater than 0.999 0.The relative standard deviations were in the range of 0.77%-4.54%and the recoveries of the analytes spiked in concentrated juices ranged from 87.20%to 105.22%.
keywords: concentrated juices free sugars high performance liquid chromatography（HPLC） differential refraction detector（RID） solid phase extraction
文章编号：201916024 中图分类号： 文献标志码：
基金项目:
| Author Name | Affiliation |
| LI Kai，ZENG Ming，SONG Hao，WANG Dong | 1.Beijing Industrial Technology Research Institute，Beijing 101111，China；2.Beijing Food Industry Research Institute，Beijing 100075，China |
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