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投稿时间：2018-10-25
投稿时间：2018-10-25
中文摘要: 建立 QuEChERS（quick、easy、cheap、effective、rugged、safe）-气相色谱-质谱联用快速测定辣椒中咪鲜胺及其代谢物2，4，6-三氯苯酚残留量的方法。样品经乙腈-乙酸（99 ∶1，体积比）提取剂后，以无水硫酸镁和N-丙基乙二胺（primary secondary amine，PSA）净化，并利用气相色谱-串联质谱进行检测。研究不同提取溶剂和无水硫酸镁PSA量（3 ∶1，质量比）对提取净化效率的影响。结果表明：在最优试验条件下，咪鲜胺及其代谢物2，4，6-三氯苯酚在0.01 μg/mL～1.00 μg/mL 范围内线性关系均良好，相关系数分别为 0.999 1 和 0.999 2，检出限为 0.003 3 mg/kg；在 0.02、0.10、0.50 μg/mL 3 个添加水平下平均回收率为96.70%～100.64%，相对标准偏差（n=7）为2.33%～4.28%。该方法具有快速、便捷、安全、清洁和成本低的特点，可用于辣椒中咪鲜胺及其代谢物2，4，6-三氯苯酚残留的快速测定。
中文关键词: QuEChERS 气相色谱-串联质谱 咪鲜胺 2，4，6-三氯苯酚 辣椒
Abstract:To develop a rapid method for the determination of residues of prochloraz and its metabolite 2，4，6-trichlorophenol in pepper by QuEChERS（quick，easy，cheap，effective，rugged，safe）-gas chromatography-mass spectrometry（GC-MS）.The samples were extracted by acetonitrile-acetic acid（99 ∶1，volume ratio），purified by anhydrous magnesium sulfate and primary secondary amine（PSA），and determined by GC-MS.Effects of different extraction solvents and anhydrous magnesium sulfate PSA （3 ∶1，mass ratio）on the determination of prochloraz and its metabolite 2，4，6-trichlorophenol were investigated.The results showed that prochloraz and its metabolite 2，4，6-trichlorophenol had a good linearity in the range of 0.01 μg/mL-1.00 μg/mL，the correlation coefficients were 0.999 1 and 0.999 2 under the optimal conditions.The limit of detection for prochloraz and its metabolite 2，4，6-trichlorophenol were 0.003 3 mg/kg.Their average recoveries at spiked levels of 0.02，0.10 and 0.50 μg/mL were 96.70%-100.64%with relative standard deviations（n=7）of 2.33%-4.28%.Therefore，the advantages of this method were simple，efficient，quick，convenient，safe，clean，lowcost and could be suitable for the analysis of prochloraz and 2，4，6-trichlorophenol residues in pepper.
文章编号：201916018 中图分类号： 文献标志码：
基金项目:甘肃省科技重大专项计划项目（17ZD2WA003）；甘肃省国际科技合作计划项目（1604WKCA001）；对发展中国家援助项目（KY201501005）；甘肃省重点实验室专项项目（1606RTSA337）；甘肃省科技创新平台专项项目（1605JTCA011）；甘肃省重点实验室建设计划（1309RTSA025）；青海省科技计划项目（2017-HZ809）
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