Abstract:To study the biological activities of ethanol extract from leaves of Morus nigra L.in Xinjiang，the crude powder of dry leaves was extracted with 80%ethanol at 30℃.The extract was dissolved and suspended in H2O and partitioned with petroleum ether，ethyl acetate and n-butanol successively and then concentrated to dry.The petroleum ether phase，ethyl acetate phase，n-butanol phase and water phase were obtained respectively.The anti-tumor activity of the extracts was studied on Hela cells by MTT assay，the antioxidant activity of the extracts was evaluated by DPPH free radicals and the hypoglycemic activity of the extracts was investigated by alpha-glucosidase inhibition model in vitro.The anti-tumor ability was from strong to weak：ethyl acetate ＞ nbutanol ＞water phase.Each phase had certain antioxidant capacity and the antioxidant capacity from strong to weak was：ethyl acetate phase ＞ n-butanol phase ＞ petroleum ether phase ＞ water phase.The hypoglycemic ability was as follows：ethyl acetate phase ＞ n-butanol ＞ water phase ＞ petroleum ether phase.Ethyl acetate phase had good antitumor，antioxidant and hypoglycemic bioactivities compared with other three parts of ethanol extract from leaves of Morus nigra L..