Abstract:Estrogen are a class of steroid chemicals that are widely found in the environment，which not only impacts the reproduction and metabolism of human and animals directly，but interferes with the synthesis，release，operation，metabolism，and combination of normal secretions after entering the body.Moreover，estrogen may cause serious damage to the reproductive and nervous system and immune system of animals and humans with low concentration.Studies have shown that estrogen active substances are widely found in milk products，domestic water and industrial water sources.The review focused on the research progress and application of genetically modified organism bioreactor detection methods，as well as the development and application of high performance liquid chromatography，gas chromatography and other detection methods in estrogen detection.