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投稿时间：2019-05-24
投稿时间：2019-05-24
中文摘要: 通过高效液相色谱法（high performance liquid chromatography，HPLC）测定食品合成着色剂的条件的研究，得出高效液相色谱法同时测定9 种合成着色剂的最佳条件。试验发现，利用固相萃取柱快速检测食品中的合成色素，可同时测定9 种色素（新红、柠檬黄、苋菜红、靛蓝、胭脂红、日落黄、亮蓝、赤藓红、诱惑红），经过甲醇和0.02 mol/L 乙酸铵梯度洗脱，流速1.0 mL/min 时，采用紫外检测器，波长254 nm 下进行检测。通过加标回收，计算回收率、相对标准偏差，各组分浓度与峰面积呈现良好的线性关系。通过对多种类型食品的实际检测，验证该方法可作为食品检测9 种合成色素的检测方法。
Abstract:It cloud get the best conditions for simultaneous determination of 9 synthetic colorants，by the research of high performance liquid chromatography （HPLC）determining synthetic colorants.The experiment showed it cloud determinate 9 kinds of pigment （red，tartrazine，amaranth，indigo，carmine，sunset yellow，bright blue，erythrosine，temptation red），using solid phase extraction column rapid detection of synthetic pigment in food ，with methanol and 0.02 mol/L ammonium acetate gradient elution，and the flow rate of 1.0 mL/min，with ultraviolet detector，detection under the wavelength of 254 nm.Through standard recovery，recovery rate and relative standard deviation，the concentration of each component had a good linear relationship with the peak area.Through the actual detection of various types of food，it verified that this method can be used as the detection method for 9 kinds of synthetic pigments in food detection.
keywords: synthetic pigment solid phase extraction high performance liquid chromatography（HPLC） food testing application
文章编号：201914034 中图分类号： 文献标志码：
基金项目:
| Author Name | Affiliation |
| SHAN Rui，GAO Yan，DU Lan-wei，ZHANG Hong | Tianjin Food Research Institute Co.，Ltd.，Tianjin 301609，China |
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