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投稿时间：2018-12-20
投稿时间：2018-12-20
中文摘要: 以藕皮为原料提取多酚，通过单因素试验分析乙醇浓度、料液比、提取温度、提取时间4 个因素对莲藕皮多酚提取得率的影响，利用正交试验对提取工艺进行优化，并以抗坏血酸为对比以羟基自由基、DPPH 自由基、超氧阴离子自由基的清除能力为指标，分析藕皮多酚提取物的体外抗氧化活性。结果表明：藕皮多酚提取优化工艺条件为：乙醇浓度为40%，料液比为1∶30（g/mL），提取温度为90 ℃，提取时间2 h，该条件下藕皮多酚类物质的提取得率为（4.45±0.05）mg/g。藕皮多酚提取物对羟基自由基、DPPH 自由基、超氧阴离子自由基消除率最高分别可达92.45%、82.11%、80.41%，证实藕皮多酚提取物具有很好的抗氧化能力。
Abstract:Lotus root skin was used as material to extra polyphenol，the effect of concentration of ethanol，the ratio of solid-liquid，the extraction temperature and the extraction time on polyphenol extraction yield were researched by single factor experiment，then the extraction process was optimized by orthogonal experiment.Whereafter，the antioxidant properties in vitro was tested by measuring hydroxyl radical scavenging activity，DPPH radical scavenging activity and superoxide radical scavenging activity compare to ascorbic acid.The result showed that the polyphenol extraction yield could be reached to （4.45±0.05）mg/g under the optimized processing as following：the concentration of ethanol was 40%，the ratio of solid-liquid was 1∶30（g/mL），the temperature of extraction was 90 ℃，the time of extraction was 2 h.The hydroxyl radical scavenging ratio，DPPH radical scavenging ratio and superoxide radical scavenging ratio could reached to 92.45 %，82.11 %，80.41 % at most respectively，antioxidant experiment result showed that the lotus root skin polyphenol extract had good antioxidant properties.
keywords: lotus root skin polyphenols extraction orthogonal experiment design antioxidant properties
文章编号：201914018 中图分类号： 文献标志码：
基金项目:江苏省农业科技自主创新资金项目（CX（17）3035）；金陵科技学院高等教育人才培养创新实验基地建设项目（基于教师科研项目食品科学类专业创新人才培养创新实验基地）；金陵科技学院校级“创客”虚拟班建设项目（2017ck009、2017ck008）
| Author Name | Affiliation |
| YAO Hong-liang，SHEN Jie，NAI Yi-fan | Food Science Department，Jinling Institute of Technology，Nanjing 210038，Jiangsu，China |
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