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投稿时间：2019-01-06
投稿时间：2019-01-06
中文摘要: 研究金纳米花作为信号放大用于黄曲霉毒素M1高灵敏检测。用粒径为（91.8±0.8）nm、分支数多的金纳米花（gold nanoflowers，AuNFs）标记黄曲霉毒素M1（aflatoxin M1，AFM1）单克隆抗体（monoclonal antibody，Mab），制备用于检测牛奶中AFM1的金纳米花免疫层析检测卡（aflatoxin M1-gold nanoflower immunochromatographic test card，AFM1-GFICT）。纳米金免疫分析读数仪被用于读取AFM1系列浓度（20 pg/mL～1 000 pg/mL）的信号值。以AFM1浓度为横坐标，信号值为纵坐标绘制非线性四参数拟合曲线。非线性四参数拟合曲线相关系数R2=0.999 3，检测限为12.3 pg/mL。检测在空白牛奶样品加入AFM1标准品浓度为0.1、0.3、0.5 μg/kg 的回收率为80.4%～118.2%，变异系数（n=10）为4.27%～9.43%。15 份牛奶样品的AFM1-GFICT 和商业化AFM1-ELISA 检测结果相比，线性拟合相关系数R2=0.976 8。
中文关键词: 金纳米花 黄曲霉毒素M1 免疫层析 非线性四参数曲线拟合
Abstract:Gold nanoflowers were studied as signal amplification for high sensitivity detection of aflatoxin M1.The conditions of the monoclonal antibody（Mab）of aflatoxin M1（AFM1）labeled with gold nanoflowers（AuNFs）with a particle size of （91.8±0.8）nm and a large number of branches were optimized.Aflatoxin M1-gold nanoflower immunochromatographic test card （AFM1-GFICT）was prepared for the detection of AFM1 in milk.A nonlinear four-parameter fit curve between the AFM1series concentration （20 pg/mL～1 000 pg/mL）and its corresponding signal value was established.The correlation coefficient was R2=0.999 3 and the detection limit was 12.3 pg/mL.The recovery of blank milk samples with AFM1standard concentration of 0.1，0.3，0.5 μg/kg was measured from 80.4%to 118.2%，and the coefficient of variation（n=10）was from 4.27%to 9.43%.15 milk samples were detected by AFM1-GFICT，and the results were compared with the results of commercial AFM1-ELISA.The correlation coefficient was R2=0.976 8.
keywords: gold nano flowers aflatoxin M1 immunochromatography nonlinear four parameters curve fitting
文章编号：201913027 中图分类号： 文献标志码：
基金项目:国家自然科学基金（81360429）；江西省教育厅科技项目（KJLD14067）
| Author Name | Affiliation |
| JIN Yu，LIU Ren-rong*，QIU Xue-mei | School of Life Science，Jiangxi Science & Technology Normal University，Nanchang 330013，Jiangxi，China |
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