Abstract:Using fresh Dendrobium officinale pressed juice as the object，the effect of different processing methods on the total alkaloid dissolution was studied.The results showed that freezing pretreatment and ultrasonic-assisted extraction had great influence on the total alkaloid dissolution.The cut fresh Dendrobium officinale was frozen and squeezed in the presence of 30 times acid water（pH 4.0，40 ℃），then the above juice was treated with 400 W ultrasonication for 10 min.Therefore，the final juice had 10.24 g/g total alkaloid dissolution，which was 94.68%higher than that of the conventional juice（5.26 μg/g）.After the pasteurization（80 ℃，30 min）was exerted on the above ultrasonic-assisted juice，the total alkaloid dissolution decreased 8.00 %，but its cold storage period could be prolonged to 6 weeks-7 weeks.Also，the total alkaloid dissolution was still 52.47%higher than that of conventional juice even if refrigerated for 7 weeks.These studies promoted the rational utilization of Dendrobium officinale resources and provided technical support for the development of Dendrobium officinale health juice.