本文已被:浏览 1359次 下载 323次
投稿时间:2018-11-04
投稿时间:2018-11-04
中文摘要: 大豆异黄酮的生理活性主要靠异黄酮苷元来发挥,该试验研究在豆浆中添加苦杏仁β-葡萄糖苷酶对大豆异黄酮苷元的影响。采取高效液相色谱检测的方法,通过响应面优化试验,得到最佳组合为:加酶量为1.4 U/5mL,酶解时间 40 min,酶解温度 46.0 ℃。测得染料木素(Ge)、黄豆黄素(Gle)、大豆苷元(De)的含量分别为(38.643±2.03)、(5.119±0.275)、(36.954±1.67)μg/mL。
中文关键词: 大豆异黄酮 大豆异黄酮苷元 高效液相色谱 豆浆 苦杏仁β-葡萄糖苷酶
Abstract:The physiological activity of soy isoflavones mainly depends on isoflavone aglycones,and the study explored the increasing of soybean isoflavone aglycones in soymilk by adding bitter almond β-glucosidase.The results of surface optimization test showed the best combination of hydrolytic technology were enzymic addition of 1.4 U/5 mL,reactive time of 40 min and reactive temperature of 46.0 ℃ by high performance liquid chromatography (HPLC)detection method,and the concentration of genistein,glycitein and daidzein were(38.643±2.03),(5.119±0.275),(36.954±1.67)μg/mL.
keywords: soybean isoflavone isoflavone aglycones high performance liquid chromatography(HPLC) soymilk bitter almondβ-glucosidase
文章编号:201912014 中图分类号: 文献标志码:
基金项目:国家自然科学基金(31460407)
引用文本: