Abstract:In order to improve the utilization value of Arctium lappa L.waste，the polysaccharides produced by solid culture of Arctium lappa L.and Ganoderma lucidum were studied，and the effect of polysaccharide on chronic inflammatory pain in mice was investigated.The Arctium lappa L.peel was used as the medium of solid culture of Ganoderma lucidum，by optimization of single factor experiment and response surface.The technological conditions of bottling amount，slice size，the ratio of liquid to solid were determined.The polysaccharides content of the Arctium lappa L.peel-Ganoderma lucidum were extracted by water and determined after extraction，alcohol precipitation and phenol sulfuric acid method.After Deproteinization，decoloration and dialysis of polysaccharides，abdominal cavity was injected of chronic inflammatory pain in mice with the injection of saline as control.The experimental results showed that the best culture process was liquid solid ratio 0.5 ∶1（mL/g），the volume of the bottle 0.2 g/mL，the size of the slice 0.7 cm2，and the content of polysaccharides were 21.87 mg/g.By statistical analysis，after injection of 130 mg/mL polysaccharide for three days，90，110 mg/mL and 130 mg/mL polysaccharides were injected for five days，there was a statistical difference in body mass between mice and other groups （P<0.05）.The injection of 5，10，30，50，70 mg/mL polysaccharides had a statistical effect on alleviating the inflammatory response to the foot of the mice.The effect was better than the injection of 90 and 110 mg/mL polysaccharides.The injection of high concentration of polysaccharide，130 mg/mL，did not affect the response to the inflammatory pain in the foot of the mice.