Abstract:Taurine in energy drinks and oral liquid was determined by pre-column derivatization with dansyl chloride.The derivatives were detected by high performance liquid chromatography（HPLC）.HPLC with variable wavelength detector was selected，and the wavelength was set at 254 nm.C18 column was used to separate the derivatives，and the sodium acetate solution and acetonitrile （30∶70，volume ratio）was used as mobile phase for isometric elution.The column temperature was set at 25 ℃and the sample injection volume was 5 μL.When the concentration of taurine was 5 μg/mL-50 μg/L，the peak area of taurine derivatives had a good linear relationship with the concentration and the correlation coefficient was 0.999.The combination of single factor and orthogonal experiment was used to optimize the derivatization experiment conditions.The results of the test had shown that the peak area of the derivative reached the maximum when derivatization temperature was 35 ℃，derivatization time was 35 min，the volume of derivatization reagent was 300 μL and the pH value of the derivative solution was 9.5.Under the experimental conditions，the relative standard deviation（RSD）of the peak area of the six groups was 1.4 % and the repeatability was good.The content of taurine in energy drinks and oral liquid was detected，and the recovery rate was 90.0%-108.0%.The deviation between the measured value and the identified value was -10.0 %-+10.0 %，the deviation of the parallel test is less than 3.0%.Therefore，this method had the advantages of easy preparation and mild reaction conditions.