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投稿时间:2018-09-03
投稿时间:2018-09-03
中文摘要: 以柑橘皮为原料,通过单因素及正交试验优化石灰水法提取柑橘皮黄酮的工艺条件,同时对石灰水提取法、NaOH 提取法和乙醇提取法的黄酮得率以及黄酮提取液中的果胶含量进行比较。单因素及正交试验结果表明:以稀释4 倍的饱和石灰水为提取剂,液料比为30 mL/g、90 ℃水浴2 h,黄酮得率最高为(5.15±0.03)mg/g。不同提取法的比较结果表明:石灰水法的黄酮得率与NaOH 法相当,高于乙醇法;石灰水法的黄酮提取液中果胶略高于乙醇法,但远低于NaOH 法。石灰水法不仅能有效地提取柑橘皮黄酮,也可以降低对杂质果胶的提取率。
Abstract:Using citrus peel as raw material,single factor and orthogonal tests were carried out to optimize citrus peel flavonoids extraction conditions of lime water extraction method.Flavonoid extraction rate and pectin content of flavonoid extract were compared among lime water,NaOH and ethanol extraction method.The results of single factor- and orthogonal test showed that:using saturated lime water diluted four times as solvent,liquid-powder ratio 30 mL/g,90 ℃ water bath processing for 2 h,flavonoids extraction rate was the highest,reached (5.15±0.03)mg/g.The results of comparison of different extraction methods showed that,flavonoids extraction rate of lime water-extraction was approximately equal to NaOH extraction,and higher than ethanol extraction;the pectin extraction of lime water-method was a little higher than ethanol extraction,but much lower than NaOH extraction.Therefore,the lime water extraction method could not only extract the citrus peel flavonoids effectively,but also reduce the extraction rate of pectin.
文章编号:201910018 中图分类号: 文献标志码:
基金项目:湖北省教育厅科学研究项目(B2017368);湖北省大学生科技创新项目(201714035013)
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