Abstract:The effect on the three contents of jujuboside A，B and spinosin in seeds of Ziziphus jujuba var.Spinosa were detected during its sprout period by high performance liquid chromatography（HPLC）.The content of jujuboside A decreased significantly with the prolongation of sprout time，jujuboside B increased significantly and reached its peak on the 7th day of sprout，then decreased slightly，spinosin decreased at first，then increased to the level of the original medicinal material （6th day of sprout），and then decreased.It showed that sprout could result in different levels of contents changed for jujuboside A，B and spinosin and had a certain re－lationship with the germination time.It provided experimental basis for the quality control of Ziziphus jujuba var.Spinosa sprouts.