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投稿时间：2018-09-19
投稿时间：2018-09-19
中文摘要: 采用正交试验法优化罗汉菜籽总黄酮提取工艺，最终确定的最佳提取条件为：乙醇体积浓度60 %，料液比1 ∶30（g/mL），提取时间为75 min，提取次数2 次，所得总黄酮粗提物含量为10.3 mg/g。采用制备色谱对总黄酮粗提物进行纯化，将提取含量由10.3 mg/g 提升至563.8 mg/g。对总黄酮粗提物和纯化物进行体外抗氧化活性研究，包括对1，1-二苯基-2-三硝基苯肼（DPPH）自由基和2，2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）二铵盐阳离子（ABTS+）自由基清除能力的测定及铁还原能力（ferric ion reducing antioxidant power，FRAP）的测定，结果表明粗提物和纯化物都有较好的抗氧化活性，且纯化后效果更显著。
Abstract:Orthogonal experimental design was used to extract flavonoids from Thlaspi Arvense Linn seeds，and the optimized extraction conditions were as follows：using 60%ethanol as solvent，the ratio of material to liquid was 1 ∶30（g/mL），extraction time was 75 min for twice.And under this extraction condition，the concentration of flavonoids was 10.3 mg/g. Preparative chromatography was also applied to purify flavonoids，and the purity raised to 563.8 mg/g from 10.3 mg/g. Different free radical antioxidant experiments，including DPPH，ABTS+and FRAP，were applied to determine the antioxidant effect of crude and purified flavonoids.The results showed that both crude and purified flavonoids exhibited high antioxidant activity，and purified flavonoids had a better effect than crude flavonoids.
keywords: Thlaspi Arvense Linn seeds flavonoids isolation purification antioxidant activity in vitro
文章编号：201908013 中图分类号： 文献标志码：
基金项目:上海市科委科研计划项目（13391900500）
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