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投稿时间：2018-11-14
投稿时间：2018-11-14
中文摘要: 结合单因素试验和响应面法优化产乳酸芽孢杆菌发酵时间、发酵液添加量、护色时间对山药多糖得率和山药相对褐变度的影响，并对比该护色液和亚硫酸钠护色液对于山药中酶促褐变和山药多糖免疫活性的影响。结果表明，最佳护色工艺为采用添加10%发酵时间为34 h 的产乳酸芽孢杆菌发酵液配制的护色液、护色时间为1 h，在此条件下，多糖得率为12.44%，相对褐变度为43.05%。该护色工艺能够有效抑制山药中多酚氧化酶（polyphenol oxidase，PPO）和过氧化物酶（peroxidase，POD）活性。该工艺护色后的山药多糖刺激小鼠巨噬细胞RAW264.7 分泌肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）、白细胞介素1（interleukin-1、IL-1β）的水平显著高于传统亚硫酸钠护色后的山药多糖。说明，采用该工艺护色后山药多糖的免疫活性显著优于采用亚硫酸钠护色后的山药多糖。
中文关键词: 山药 多糖 护色 免疫调节 RAW264.7细胞
Abstract:The present study optimized the processing conditions of a fermented color-protective agent by Bacillus sp. DU-106 with response surface methodology，and analyzed its effects on the polysaccharide yield，browning index，and immunomodulatory activity of polysaccharide for Dioscorea opposita Thunb. The result showed that the optimum processing conditions of color-protective agent were obtained as follow：the fermentation time 34 h；the amount of color-protective agent 10%；soaking time 1 h，yielding rate as high as 12.44 % and the browning index only 43.05 %. This color-protective agent inhibited of polyphenol oxidase（PPO）and peroxidase （POD）activity. The secretion levels of TNF-α and IL-1β of RAW264.7 by polysaccharide from the Dioscorea opposita Thunb by using color-protective agent were significantly higher than those of polysaccharide from the Dioscorea opposita Thunb by using sulfur color-protect，indicating polysaccharide from the Dioscorea opposita Thunb by using color-protective agent possessed higher immunomodulatory activity.
keywords: Dioscorea opposita Thunb polysaccharide color-protection immunomodulatory regulate RAW264.7 cell
文章编号：201908008 中图分类号： 文献标志码：
基金项目:广东省省级科技计划项目（2016B090918093）
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