Abstract:The antioxidant effect of pumpkin polysaccharide and ginsenoside on the liver cells of mice after hydrogen peroxide injury was studied. In vivo mouse hepatocytes were taken for H2O2 induction，and the cell oxidative stress injury model was successfully established. The cells were divided into normal group，model group，pumpkin polysaccharide group at different concentrations，ginsenoside group and combination group to investigate the antioxidant activities of each component in the cells. Experimental results showed that the separation and purification of pumpkin polysaccharide had antioxidant activity，pumpkin polysaccharide and ginseng saponin Rg1 joint，when using cell vitality enhanced obviously，than a single component in malondialdehyde（MDA），glutathione peroxidase（GSH-Px），superoxide dismutase（SOD）and catalase（CAT）index on the joint synergy，antioxidant effect was more obvious，and it mitigated the effects of oxidative damage.