Abstract:Four kinds of corresponding polysaccharides from Lentinus edodes（LEP）were obtained by 4 extraction methods：microwave extraction，ultrasonic extraction，hot water extraction and enzyme extraction.The 4 polysaccharides were characterized by high performance anion exchange chromatography and fourier transform infrared spectrometry，and selected the optimal method based on the extraction rate and antioxidant activity.Results revealed the monosaccharide components of the 4 polysaccharides were substantially the same，but their molar ratios were significantly different.The order of extraction yields of the 4 polysaccharides was as followsultrasonicextraction polysaccharide ＞microwaveextraction polysaccharide ＞enzymeextraction polysaccharide＞hot water extraction polysaccharide.Among the 4 polysaccharides，the content of microwave extraction polysaccharide was the highest（43.17%），much higher than other 3 polysaccharides，and the protein content was the lowest（3.31%）.The results of antioxidant tests showed that the scavenging power of·OH were highest，the scavenging power of DPPH·and the reducing power were comparable with other polysaccharides.Considering the extraction yield， polysaccharide content and antioxidant， microwave extraction was the best method.