Abstract:Abstract：A method was developed to analyze the stable carbon isotope ratio of six flavour characters（ethanol，glycerol，3-methyl butanol，ethyl lactate，acetic acid，2，3-butanediol）in wine using gas chromatography combustion isotope ratio mass spectrometer（GC-C-IRMS）.After filtration by millipore membrane，the wine or diluted wine was injected into GC-C-IRMS.The six flavour characters was seperated completely and the analytical time of each run was less than 20 min.The precision of this method was 0.064‰-0.227‰for analyzing standards and 0.115‰-0.300‰for wine samples.The carbon isotope ratioes of six volatile components were measured using GC-C-IRMS.The results indicated that there was a degree of difference in different countries and in components of the same sample.