Abstract:This article reviewed the detection methods of casein phosphopeptides in food，including high performance liquid chromatography，high performance liquid chromatography-mass spectrometry，capillary zone elect rophoresis and Barium-ethanol precipitation，etc.The advantages and disadvantages of several detection methods and the difficulties in the detection process were introduced.The research data showed that high performance liquid chromatography had the characteristics of simple operation，rapidity and high sensitivity，and was widely used in many fields.It was considered that the detection method of casein phosphopeptide should be based on high performance liquid chromatography to explore a more applicable detection method.The present paper could provided a powerful reference for the establishment of a national standard detection method for the uniform casein phosphopeptides in food.