Abstract:The prebiotic effect of Pholiota nameko peptides on Bifidobacterium adolescent in vitro was studied in this paper.Different concentrations of P.nameko peptides were added into the liquid fermentation medium of B.adolescent，and the viable counts during the fermentation and storage process were determined.The results showed that the growth of B.adolescent was promoted when the concentration of P.nameko peptides varied from 0.5 g/L to 4.0 g/L，and the highest viable count was 1.78×109cfu/mL after 12 h fermentation，which was 2.88 times as higher as that of the control group （P＜0.05）.The tolerance of B.adolescent to acid environment was enhanced by addition of P.nameko peptides，and the viable count remained above 80%after storage at 4℃ for 28 d.When the addition was 1.0 g/L，the growth promoting effect of P.nameko peptides on B.adolescent was higher than that of fructooligosaccharides，and the final viable count was 1.18 ×109cfu/mL after 12 h fermentation，which was 2.04 times as higher as that of the control group （P＜0.05）.P.nameko peptides could promote the growth of B.adolescent，and possess potential application value in B.adolescent products as bifidus factors.